Application of multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI) for analysis of chromosomal passenger protein function

Author

Harada, Yoshinori ; Dai, Ping ; Takamatsu, Tetsuro

Author_Institution

Dept. of Pathology & Cell Regul., Kyoto Prefectural Univ. of Med., Kyoto

fYear

2008

fDate

6-9 Nov. 2008

Firstpage

352

Lastpage

354

Abstract

Recently, we have developed a technology which can specifically inactivate intracellular protein molecules with high spatial and time resolution by using femtosecond pulse laser and green fluorescent protein, i.e., multiphoton excitation-evoked chromophore-assisted laser inactivation (MP-CALI). To analyze precise functions of chromosomal passenger proteins, we conduct experiments by applying the method of MP-CALI.

Keywords

biological effects of laser radiation; cellular biophysics; fluorescence; genetics; molecular biophysics; multiphoton processes; proteins; MP-CALI; chromosomal passenger protein function; femtosecond pulse laser; green fluorescent protein; high spatial resolution; intracellular protein molecules; multiphoton excitation-evoked chromophore-assisted laser inactivation; time resolution; Biological cells; Fluorescence; Laser excitation; Microscopy; Network address translation; Optical wavelength conversion; Power lasers; Protein engineering; Spatial resolution; Ultrafast optics;

fLanguage

English

Publisher

ieee

Conference_Titel

Micro-NanoMechatronics and Human Science, 2008. MHS 2008. International Symposium on

Conference_Location

Nagoya

Print_ISBN

978-1-4244-2918-9

Electronic_ISBN

978-1-4244-2919-6

Type

conf

DOI

10.1109/MHS.2008.4752476

Filename

4752476