Intervention of cardiomyocyte death based on the impedance-sensing technique of monitoring cell adhesion

Cardiomyocyte death is a critical risk factor in a variety of cardiovascular diseases. At the level of individual cardiomyocytes, cell adhesion alters at the early phase of ongoing death. It suggested the possibility of detecting the early sign of cell death through cell detachment and so that we could intervene in the cell response before cell fell into step with the irreversible death processes. In this work, we demonstrated the application of our impedance-sensing technique in the intervention of cell responses to chemical stimuli. The results showed that the cell detachment could be detected with our impedance-sensing technique earlier than conventional biochemical staining techniques, and it could be used to guide the precautionary removal of TNF-alpha from the cell culture to intervene in the cell responses and prevent cardiomyocytes from cell death.