Title :
Label-free analysis of DNA methylation using optofluidic ring resonators
Author :
Suter, Jonathan D. ; Howard, Daniel J. ; Shi, Huidong ; Caldwell, Charles W. ; Fan, Xudong
Author_Institution :
Dept. of Biol. Eng., Univ. of Missouri, Columbia, MO, USA
Abstract :
We demonstrate the utility of the opto-fluidic ring resonator (OFRR) sensor for analyzing methylated oligonucleotides. Cytosine methylation, a regular epigenetic function in cellular growth and metabolism, may have ties to abnormal suppression of key genes involved with cellular proliferation. Such behavior is suspected to be strongly related to the occurrence of several types of cancers. The OFRR is demonstrated as a tool both for detecting DNA hybridization and methylated cytosines residues.
Keywords :
DNA; bioMEMS; biochemistry; biosensors; microfluidics; micromechanical resonators; DNA methylation; cancer; cellular growth; cellular proliferation; cytosine methylation; gene suppression; label free analysis; metabolism; oligonucleotides; optofluidic ring resonators sensor; Animals; Cell Proliferation; Cytosine; DNA Methylation; Epigenesis, Genetic; Equipment Design; Humans; Methylation; Mice; Neoplasms; Nucleic Acid Hybridization; Oligonucleotides; Optics and Photonics;
Conference_Titel :
Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE
Conference_Location :
Minneapolis, MN
Print_ISBN :
978-1-4244-3296-7
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2009.5333866
