Title :
Dual compartment neurofluidic system for electrophysiological measurements in physically isolated neuronal cell cultures
Author :
Kanagasabapathi, Thirukumaran T ; Wang, Ke ; Mellacé, Marco ; Ramakers, Ger J A ; Decré, Michel M J
Author_Institution :
Dept. of Minimally Invasive Healthcare, Philips Res. Labs. Eindhoven, Eindhoven, Netherlands
Abstract :
This work investigates an approach to record electrophysiological measurements of neuronal cell cultures in a dual compartment neurofluidic system. The two compartments are separated by 10-mum-wide and 3-mum-high microchannels and this provides a physical isolation of neurons allowing only neurites to grow between the compartments. We present long-term cell viability in closed compartment devices, neurite growth across the microchannels and a recording setup for the long-term recording of the network activity over 21 Days-in-Vitro (DIV). Structural and fluidic isolation between the compartments are demonstrated using transfection experiments and neurotoxin exposure, respectively.
Keywords :
bioelectric phenomena; cellular biophysics; neurophysiology; dual compartment neurofluidic system; electrophysiology; fluidic isolation; long-term cell viability; neurites; neurotoxin exposure; physically isolated neuronal cell culture; structural isolation; transfection experiment; Cells, Cultured; Microfluidics; Neurons;
Conference_Titel :
Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE
Conference_Location :
Minneapolis, MN
Print_ISBN :
978-1-4244-3296-7
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2009.5333892