Title :
Dissolved core alginate microspheres as “smart-tattoo” glucose sensors
Author :
Chaudhary, Ayesha ; Raina, Monica ; McShane, Michael J. ; Srivastava, Rohit
Author_Institution :
Sch. of Biosci. & Bioeng., Indian Inst. of Technol. Bombay, Mumbai, India
Abstract :
The feasibility of multilayer thin film coated dissolved-core alginate-templated microsphere sensors based on fluorescence resonance energy transfer and competitive binding, was explored in simulated interstitial fluid, using glucose as a model analyte. The glucose sensitivity was observed to be 0.89%/mM glucose with a linear response in the range of 0-50 mM glucose. The sensor response was observed to be completely reversible in nature with a response time of 120 seconds. The system was further demonstrated to respond similarly using near-infrared dyes (Alexa Fluor-647-labeled dextran as donor and QSY-21-conjugated apo-GOx as acceptor) which exhibited a sensitivity of 0.94%/mM glucose with a linear response in range of 0-50 mM glucose, making the sensor more amenable to in vivo use, when implanted in scattering tissue.
Keywords :
biosensors; dyes; fluorescence; microsensors; molecular biophysics; multilayers; thin films; competitive binding; dissolved-core alginate-templated microsphere sensors; fluorescence resonance energy transfer; linear response; model analyte; multilayer thin film; near-infrared dyes; simulated interstitial fluid; smart-tattoo glucose sensors; Alginates; Biomedical Engineering; Biosensing Techniques; Cyclic AMP; Equipment Design; Extracellular Fluid; Fluorescence Resonance Energy Transfer; Glucose; Glucose Oxidase; Glucuronic Acid; Hexuronic Acids; Humans; Microscopy, Electron, Scanning; Microspheres; Spectrophotometry; Spectroscopy, Near-Infrared; Time Factors;
Conference_Titel :
Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE
Conference_Location :
Minneapolis, MN
Print_ISBN :
978-1-4244-3296-7
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2009.5334597