Title :
Iterative correction of suppressed peptide profiles from FTMS measurements
Author :
Ma, Xuepo ; Travis, J. Hestilow ; Cui, Jian ; Zhang, Jianqiu
Author_Institution :
Department of Electrical and Computer Engineering, University of Texas at San Antonio, San Antonio, TX, USA
Abstract :
Analysis of peptide profiles from a Liquid Chromatography Fourier Transform Mass Spectrometry (LC/FTMS) measurement reveals a non-linear distortion in intensity. Investigation of the measured CVi /C12 ratios comparing with theoretical ones shows that the non-linearity can be attributed to low intensity signal suppression of low abundance peptide peaks. We find that the suppression is homogenous for different isotopes of identical peptides but non-homogenous for different peptides. We developed an iterative correction algorithm that corrects the intensity distortions for peptides with relatively high abundance. This algorithm can be applied in a wide range of applications using FTMS.
Keywords :
ICM; LC/FTMS; correction; suppression;
Conference_Titel :
Bioinformatics and Biomedicine (BIBM), 2010 IEEE International Conference on
Conference_Location :
Hong Kong
Print_ISBN :
978-1-4244-8306-8
DOI :
10.1109/BIBM.2010.5706567
