Title :
Degradable polymers for gene delivery
Author :
Sunshine, Joel ; Bhise, Nupura ; Green, Jordan J.
Author_Institution :
Dept. of Biomed. Eng., Johns Hopkins Univ., Baltimore, MD, USA
Abstract :
Degradable polymers were synthesized that self-assemble with DNA to form particles that are effective for gene delivery. Small changes to polymer synthesis conditions, particle formulation conditions, and polymer structure led to significant changes to efficacy in a cell-type dependent manner. Polymers presented here are more effective than Lipofectamine 2000 or polyethylenimine for gene delivery to cancerous fibroblasts or human primary fibroblasts. These materials may be useful for cancer therapeutics and regenerative medicine.
Keywords :
DNA; biodegradable materials; cancer; cellular biophysics; gene therapy; materials preparation; polymer structure; tumours; DNA self-assembly; Lipofectamine 2000; cancer therapeutics; cancerous fibroblasts; cell-type dependent manner; degradable polymer synthesis condition; gene delivery; human primary fibroblasts; particle formulation condition; polyethylenimine; polymer structure; regenerative medicine; Animals; Biodegradation, Environmental; COS Cells; Cell Line, Tumor; Cercopithecus aethiops; Drug Carriers; Fibroblasts; Gene Therapy; Gene Transfer Techniques; Humans; Lipids; Neoplasms; Polyethyleneimine; Polymers; Regenerative Medicine;
Conference_Titel :
Engineering in Medicine and Biology Society, 2009. EMBC 2009. Annual International Conference of the IEEE
Conference_Location :
Minneapolis, MN
Print_ISBN :
978-1-4244-3296-7
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2009.5334767
