Heterogeneous Vesicle Arrays for Biosensing Applications

Microarrays play a key-role in life sciences and in diagnostics by providing a powerful tool for high throughput parallel analysis of complex samples. We present a novel approach to create heterogeneous vesicle arrays with optimized interface chemistry and low unspecific response. Here, complexes of biotinylated single-stranded DNA and streptavidin are spotted onto a non-fouling biotinylated polymer layer. The so-obtained DNA array is then transformed into a vesicle array after incubation with a solution of nanoobjects tagged with the complementary strands. Phospholipid vesicle arrays can potentially be transformed into membrane protein arrays.