DocumentCode :
2420242
Title :
ITO-coated glass/polydimethylsiloxane continuous-flow PCR chip
Author :
Joung, Seung-Ryong ; Kim, Jaewan ; Choi, Y.J. ; Kang, C.J. ; Kim, Yong-Sang
Author_Institution :
Dept. of Nano Sci. & Eng., Myongji Univ.
fYear :
2007
fDate :
16-19 Jan. 2007
Firstpage :
691
Lastpage :
694
Abstract :
We propose a continuous-flow polymerase chain reaction (PCR) chip using indium-tin-oxide (ITO)-coated glass/polydimethylsiloxane (PDMS) materials for DNA amplification. The continuous-flow PCR chip enables fast thermal cycling and series amplification, which are difficult to achieve in a conventional PCR or micro-chamber PCR chip. Six heaters of ITO thin films were fabricated on glass for the thermal cycling of the flowing PCR sample. The PDMS microchannel was fabricated using a negative molding method. The width and depth of the microchannel are 250 mum and 200 mum, respectively, with a total channel length of 1340 mm. The PCR chip can perform 20 cycles of amplifications. The ratio of the channel lengths for three different temperature zones, namely denaturation, annealing, and extension, is 2:2:3, respectively. Using the fabricated continuous-flow PCR chip, two DNA plasmids (720-bp pKS-GFP and 300-bp PG-noswsi) were successfully amplified.
Keywords :
DNA; biomolecular electronics; glass; indium compounds; microchannel flow; thin films; 1340 mm; 200 micron; 250 micron; DNA amplification; DNA plasmids; In2SnO3; PDMS microchannel; continuous-flow PCR chip; continuous-flow polymerase chain reaction chip; glass/polydimethylsiloxane materials; indium-tin-oxide thin films; indium-tin-oxide-coating; microchamber PCR chip; negative molding method; thermal cycling; Annealing; DNA; Electrodes; Glass; Indium tin oxide; Microchannel; Microfluidics; Polymer films; Resists; Temperature; PDMS; continuous-flow PCR chip; glass; indium-tin-oxide film; polymerase chain reaction;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nano/Micro Engineered and Molecular Systems, 2007. NEMS '07. 2nd IEEE International Conference on
Conference_Location :
Bangkok
Print_ISBN :
1-4244-0610-2
Type :
conf
DOI :
10.1109/NEMS.2007.352113
Filename :
4160416
Link To Document :
بازگشت