Title :
Single-Molecule Detection in Temperature-Controlled Microchannels
Author :
Wang, Bin ; Fei, Jingyi ; Gonzalez, Ruben L. ; Lin, Qiao
Author_Institution :
Dept. of Mech. Eng., Columbia Univ.
Abstract :
This paper reports a temperature-controlled microfluidic platform for single-molecule studies. Parallel microchannels are formed between glass and quartz, and are integrated with on-chip heaters and temperature sensors. Combined with surface derivatization and fluorescent labeling of biomolecules, temperature-dependent changes in the rates of fluorescence photobleaching are measured. The results demonstrate the potential of the device to provide an ideal environment for temperature-dependent single-molecule studies.
Keywords :
biomolecular electronics; glass; microchannel flow; microsensors; optical saturable absorption; quartz; temperature control; temperature sensors; fluorescence photobleaching; fluorescent biomolecule labeling; glass; microfluidic platform; on-chip heaters; parallel microchannels; quartz; single-molecule detection; surface derivatization; temperature control; temperature sensors; DNA; Fluorescence; Microchannel; Microfluidics; Micromechanical devices; Microscopy; Molecular biophysics; Photobleaching; Temperature control; Temperature sensors; microchannel; microfluidics; single-molecule detection; temperature control;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems, 2007. NEMS '07. 2nd IEEE International Conference on
Conference_Location :
Bangkok
Print_ISBN :
1-4244-0610-2
DOI :
10.1109/NEMS.2007.352180
