DocumentCode :
2422778
Title :
The application of Iron Oxide magnetic nanoparticles to improve the binding efficiency of the IgG and Thiol SAMs
Author :
Yao, Da-Jen ; Lin, Chun-Yi ; Tseng, Fangang
Author_Institution :
Dept. of Eng. & Syst. Sci., Nat. Tsing Hua Univ., Hsinchu
fYear :
2007
fDate :
16-19 Jan. 2007
Firstpage :
172
Lastpage :
175
Abstract :
Superparamagnetic nanoparticles have been fabricated successfully, which are composed of Fe3O4 by using wet chemical co-precipitate method. The nanoparticles were functionalized on the surface by conjugated with the abti-Rabbit IgG. In the recently research, we try to use this system to approach the work which is to increase the reaction efficiency between antibodies and antigens by applying magnetic fields to alternate the binding samples in solution. The applied magnetic fields are produced by electro-magnets with AC or DC input. In the preliminary experiment, the antibodies were reacted with the carboxyl group Thiol SAMs that were modified on the glass chip with gold film. The surface plasma resonance (SPR) was used to detect the binding number of antibodies on the chip. The binding efficiency could be further calculated. The results showed the binding efficiency could be improved by using superparamagnetic nanoparticles with applying magnetic fields.
Keywords :
crystal binding; iron compounds; molecular biophysics; monolayers; nanoparticles; nanotechnology; self-assembly; superparamagnetism; surface plasmon resonance; Fe3O4; Thiol SAMs; abti-rabbit IgG; improved binding efficiency; magnetic fields; superparamagnetic nanoparticles; surface plasma resonance; wet chemical co-precipitate method; Coatings; Glass; Gold; Iron; Magnetic fields; Nanoparticles; Plasmons; Resonance; Surface treatment; Systems engineering and theory; SPR; binding efficiency; nanoparticles;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Nano/Micro Engineered and Molecular Systems, 2007. NEMS '07. 2nd IEEE International Conference on
Conference_Location :
Bangkok
Print_ISBN :
1-4244-0610-2
Type :
conf
DOI :
10.1109/NEMS.2007.352255
Filename :
4160558
Link To Document :
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