Investigations of bio markers for human lymphoblastoid cells using Atomic Force Microscopy

Author

Yang, Ruiguo ; Xi, Ning ; Fung, Carmen Kar Man ; Lai, King Wai Chiu ; Seiffert-Sinha, Kristina ; Sinha, Animesh A. ; Zhang, Weijing

Author_Institution

Dept. of Electr. & Comput. Eng., Michigan State Univ., East Lansing, MI, USA

fYear

2010

fDate

20-23 Jan. 2010

Firstpage

129

Lastpage

132

Abstract

We report the use of Atomic Force Microcopy (AFM) in combination with Fluorescence Microscopy (FM) for the investigation of non-Hodgkin´s type B cells lymphoma after antibody treatment. Rituximab is used in the treatment this cancer type. It is a chimeric monoclonal antibody directed against the protein Cluster of Differentiation 20 (CD20), which is expressed on mature B cells, including the B cell lymphoma surface. We applied anti-CD20 antibody to the B lymphoma cells that was labeled with fluorophore. Simultaneous imaging was then performed by both FM and AFM. Immunofluorescence imaging confirms the binding of Rituximab to the CD20 protein; while the AFM imaging revealed changes in lymphoma cells in terms of mean cell height and cell surface roughness. In addition, our AFM based nanomanipulation system can assist in the analysis of mechanical property of the cell by recording the force-displacement curves at the cell surface. AFM imaging and measurements may provide biomarkers of cell behavior and may facilitate the treatment of lymphoma clinically as a pretest to determine the effectiveness of the drug Rituximab on lymphoma cells from individual patients.

Keywords

atomic force microscopy; biochemistry; biomechanics; biomedical optical imaging; bonds (chemical); cancer; cellular biophysics; molecular biophysics; optical microscopy; patient treatment; proteins; surface roughness; AFM; CD20 protein; Rituximab binding; antibody treatment; atomic force microscopy; biomarkers; cancer type treatment; cell height; cell surface roughness; fluorescence microscopy; fluorophore; force-displacement curves; human lymphoblastoid cells; immunofluorescence imaging; mechanical property; monoclonal antibody; nanomanipulation system; non-Hodgkin type B cell lymphoma; protein cluster-of-differentiation; AFM live cell imaging; Atomic Force Microscopy; Fluorescence Microscopy; Rituximab; cell immobilization; human lymphoblastoid cells;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2010 5th IEEE International Conference on

Conference_Location

Xiamen

Print_ISBN

978-1-4244-6543-9

Type

conf

DOI

10.1109/NEMS.2010.5592162

Filename

5592162