Immobilized polydiacetylene vesicle for label-free biosensor

The polydiacetylene vesicles-based sensors have been of interest due to their unique chromatic properties, such as blue-to-red color transition. The liposome-like vesicles were immobilized on solid substrates using various polydiacetylene derivatives synthesized with different functional groups, such as, amine, carboxylic acid, and biotin, etc. Through their fluorogenic properties, the label-free biosensor was constructed with PDA immobilized on the solid substrate. Here, we showed some results of our recent studies to construct label-free sensors for detecting protein and bacterial cells using the stress-induced fluorescence emission of PDA vesicles. From the results, we anticipate that the system of micro-patterned PDA-based biosensor can be used to fabricate a label-free diagnostic sensor.