Title :
PDMS microlens fabricated for compact disk enzyme-linked immunosorbent assay (CD-ELISA) applications
Author :
Wen Wai ; Tseng, Kuan-Ju ; Kuo, Ju-Nan
Author_Institution :
Dept. of Autom. Eng., Nat. Formosa Univ., Huwei, Taiwan
Abstract :
This paper presents a polydimethyl-siloxane (PDMS) microlens bonding on a compact disk (CD) microfluidic device that performs an enzyme-linked immunosorbent assay (ELISA) to enhance the sensitivity of bio-detection. The PDMS microlens can be fabricated with ease using soft replica molding method. A commercial software tool (ZEMAX) has been established to analyze the focal length of the microlens. A fluorescence detection system comprising of a PDMS microlens, a CD microfluidic device and an optical detection module is demonstrated. Consequently, using the developed microlens, the detection signal and the signal-to-noise ratio of the ELISA immunoassay in microfluidic devices were significantly improved. Successful detected the fluorescent-labeled beads is demonstrated using the developed method.
Keywords :
bioMEMS; biochemistry; biosensors; enzymes; fluorescence; microfabrication; microfluidics; microlenses; molecular biophysics; moulding; photodetectors; polymers; replica techniques; soft lithography; CD-ELISA applications; PDMS microlens; ZEMAX; bio-detection; commercial software tool; compact disk enzyme; enzyme-linked immunosorbent assay; fluorescence detection system; fluorescent-labeled beads; microfluidic device; optical detection module; polydimethyl-siloxane; signal-to-noise ratio; soft replica molding method; Bio-detection; ELISA; Microfluidic device; Microlens;
Conference_Titel :
Nano/Micro Engineered and Molecular Systems (NEMS), 2010 5th IEEE International Conference on
Conference_Location :
Xiamen
Print_ISBN :
978-1-4244-6543-9
DOI :
10.1109/NEMS.2010.5592184
