Integrated micro potentiometric immunosensor for percent hemoglobin-A1C based on nano-gold multilayer

Author

Qiannan Xue ; Chao Bian ; Hong Zhang ; Shanhong Xia

Author_Institution

State Key Lab. of Transducer Technol., Chinese Acad. of Sci., Beijing, China

fYear

2010

fDate

20-23 Jan. 2010

Firstpage

573

Lastpage

577

Abstract

We present a novel method for antibodies immobilization to fabricate a percent hemoglobin-A1c micro immunosensor. Nano-gold which is introduced by a novel seed mediated growth method is used for a micro potentiometric immunosensor of hemoglobin-A1c (HbA1c). To eliminate the nonspecific sites, nano-gold is capped with mixed self-assembled monolayers (MSAMs) to immobilize the antibodies stably and efficiently. This biomimetic layer preparation method has been applied firstly for modifying micro extended gate electrode array of biologically sensitive field-effect-transistor (BioFET). And combining with standard CMOS and MEMS techniques, this immunosensor exhibited high sensitivity, good stability, and achieved multichannels detection.

Keywords

biosensors; field effect transistors; gold; nanobiotechnology; organic compounds; self-assembly; BioFET; CMOS technique; MEMS technique; antibodies immobilization; biologically sensitive field effect transistor; biomimetic layer preparation; hemoglobin-A1c; micro extended gate electrode array; micropotentiometric immunosensor; nanogold multilayer; self assembled monolayer; Gold nanoparticles; Ion-sensitive field-effect transistors (ISFETs); Micro immunosensor; Mixed self-assembled monolayers; Potentiometric;

fLanguage

English

Publisher

ieee

Conference_Titel

Nano/Micro Engineered and Molecular Systems (NEMS), 2010 5th IEEE International Conference on

Conference_Location

Xiamen

Print_ISBN

978-1-4244-6543-9

Type

conf

DOI

10.1109/NEMS.2010.5592467

Filename

5592467