DocumentCode
2433696
Title
Cloning and expressing an OsDCL3b gene from Oryza sativa indica
Author
Peng, Xiaojue ; Yu, Chao ; Li, Shaobo ; Zhu, Youlin ; Chen, Zuyu
Author_Institution
Sch. of Life Sci. & Food Eng., Nanchang Univ., Nanchang, China
fYear
2011
fDate
24-26 June 2011
Firstpage
8108
Lastpage
8111
Abstract
A full length dicer-like gene was cloned from 9311 (Oryza sativa indica) by RACE (GenBank accession No: DQ208406). It should be an OsDCL3b through homogeneous alignment and locate on chromosome 10. This gene might have alternative splicing transcripts. Real-time PCR results indicated that it expressed at different levels in root, stem, leaf and flower, respectively. The highest expression level of this gene was detected in flower, while the lowest expression level was detected in root.
Keywords
DNA; botany; genetics; molecular biophysics; Oryza sativa indica; OsDCL3b gene cloning; OsDCL3b gene expression; RACE; Real-time PCR; alternative splicing transcripts; chromosome; flower; full length dicer-like gene; homogeneous alignment; leaf; root; stem; Animals; Cloning; Materials; Proteins; RNA; Real time systems; Splicing; Oryza sativa indica; OsDCL3; Real-time PCR; alternative splicing;
fLanguage
English
Publisher
ieee
Conference_Titel
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location
Nanjing
Print_ISBN
978-1-4244-9172-8
Type
conf
DOI
10.1109/RSETE.2011.5964037
Filename
5964037
Link To Document