Title :
Development fluorescent quantitative PCR method for detection Di-n-butyl phthalate in water sample
Author :
Zhao, Xiaoxiang ; Chaisuwan, Nuanapa
Author_Institution :
Coll. of Environ. Sci. & Eng., Donghua Univ., Shanghai, China
Abstract :
A fluorescence quantitative PCR method for detection of Di-n-butyl phthalate in water sample has been developed. DBP was extract from water samples using liquid-liquid extraction. The binding-DNA which includes specific primers and pUC19 as template were synthesized by conventional PCR. DBP concentration calculates from standard curve between concentrations of DBP and Ct value by SYBR Green. The lowest detection limit can be detected at 10-10 g/L. The concentration 0.1058 ug/L of DBP in water sample could be detected using this method. Results show that FQ-PCR is more sensitive with lower detection limits. The results suggest that FQ-PCR can be a method for detection and quantification of DBP in water.
Keywords :
biotechnology; microorganisms; Di-n-butyl phthalate detection; SYBR Green; binding-DNA; fluorescent quantitative PCR method; liquid-liquid extraction; polymerase chain reaction; Correlation; DNA; Equations; Erbium; Fluorescence; Instruments; Water pollution; Di-n-butyl phthalate; EXOIII; Fluorescent Quantitative PCR; HPLC;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5964061
