A microfluidic device for single and small population cell trapping and lysis of Pseudo-nitzschia

We present a microfluidic device that is capable of trapping single and small populations of Pseduo-nitzschia for subsequent cell lysis and domolic acid (DA) extraction. Microcages are integrated within a microchannel to capture the cells and hydrodynamic focusing is utilized to guide the cells into the cages. The microchip is coupled with an ultrasonication device, which enables for rapid cell lysis. Our results demonstrate effective trapping of single and multiple cells of various sizes, which can be lysed 10x faster than conventional methods. This device provides a simple and efficient tool for studying the biological mechanisms of DA production by Pseudo-nitzschia, which is crucial for minimizing the threat of amnesic shellfish poisoning. Moreover, the portability of this device allows for the integration of on-chip components and sensors enabling for in field DA detection.