Title :
The novel mTOR inhibitor RAD001 (Eeverolimus) induces antiproliferative effects in goat fetal fibroblasts
Author :
Wu, Manlin ; Chen, Yuhao ; Fu, Haixia ; Liu, Bingchun ; Cui, Xinjie ; Li, Shuyu ; Wang, Zhigang
Author_Institution :
Coll. of Life Sci., Inner Mongolia Univ., Hohhot, China
Abstract :
The mammalian target of rapamycin (mTOR) is an important controller of cell proliferation. RAD001 (everolimus) is a specific inhibitor of mTOR, can block mTOR signaling pathway. The purposes of this study are to explore the inhibitory effects of RAD001 on Cashmere goat fetal fibroblasts (GFb cells) proliferation. We examined both the effects of RAD001 on cell proliferation and cytostatic effect of GFb cells. The relative viability of the cells treated with RAD001 over 0.33 μM was significantly different compared with that of control (P <; 0.01). As a result, RAD001 induced a dose-dependent decrease in cell proliferation. Taken together, these data showed that RAD001 can inhibit mTOR signaling and proliferation in GFb cells in vitro.
Keywords :
cellular biophysics; drugs; enzymes; molecular biophysics; Cashmere goat fetal fibroblasts; Everolimus; antiproliferative effects; cell proliferation; cell viability; cytostatic effect; inhibitory effects; mTOR inhibitor RAD001; mTOR signaling pathway; mammalian target of rapamycin; Cancer; Drugs; Fibroblasts; Inhibitors; Mice; Proteins; Tumors; RAD001; goat fetal fibroblasts; mTOR; proliferation;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5964203
