A high-sensitivity CMOS-compatible immunosensor based on the HRP/TMB/streptavidin system

Author

Tai, Yu-Ting ; Chang, Yu-Wei ; Lin, Ming-Yu ; Yang, Yuh-Shyong ; Huang, Yang-Tung

Author_Institution

Inst. of Biochem. Eng., Nat. Chiao Tung Univ., Hsinchu

fYear

2008

fDate

Nov. 30 2008-Dec. 3 2008

Firstpage

297

Lastpage

300

Abstract

In this report, a CMOS-compatible colorimetric biosensor based on HRP/TMB/streptavidin system is proposed. HRP activity is used for labelled target quantification by photometric detector. The modulation of light intensity due to enzyme reaction can easily be detected in 96 - well microarray plate by providing 650 nm light source (LED). There is good correlation between absorbance in 650 nm and the concentration of HRP - streptavidin with detection limit in 1.33 pM. This device is reusable and suits for various targets by microarray plate modification rather than biochip. With the advantage of portable, low cost, and reusable, this new designed immunosensor could be widely used for both research and diagnostic purposes.

Keywords

CMOS integrated circuits; biosensors; colorimetry; detector circuits; enzymes; light emitting diodes; spectrophotometry; CMOS-compatible colorimetric biosensor; HRP/TMB/streptavidin system; enzyme reaction; high-sensitivity CMOS-compatible immunosensor; horseradish peroxidase; labelled target quantification; light emitting diode; light intensity; microarray plate; photometric detector; wavelength 650 nm; Biochemistry; Biosensors; Costs; Detectors; Immune system; Intensity modulation; Light emitting diodes; Light sources; Optical modulation; Photometry; CMOS integrated circuits; horseradish peroxidase; immunosensor;

fLanguage

English

Publisher

ieee

Conference_Titel

Sensing Technology, 2008. ICST 2008. 3rd International Conference on

Conference_Location

Tainan

Print_ISBN

978-1-4244-2176-3

Electronic_ISBN

978-1-4244-2177-0

Type

conf

DOI

10.1109/ICSENST.2008.4757117

Filename

4757117