Computer Assisted Parental Sequences Analysis as a Previous Step to DNA Shuffling Process

Experiments of directed evolution have been successfully used to improve specific biological functions. DNA shuffling is a directed evolution process which generates genetic diversity through the recombination of parental sequences. It is expected that the sequences resulting from a Shuffling process be a mixture of the parental sequences. In order to evaluate which pair of sequences could potentially produce the best result, this paper analyses, using information given by a software, the adequacy of three pairs of parental sequences as candidates for undergoing a DNA shuffling process.