Title :
Analysis and design of a versatile synthetic network for inducible gene expression in mammalian systems
Author :
Polynikis, A. ; Cuccato, G. ; Criscuolo, S. ; Hogan, S.J. ; di Bernardo, M. ; Di Bernardo, D.
Author_Institution :
Dept. of Eng. Math., Univ. of Bristol, Bristol, UK
Abstract :
This paper presents a mathematical model for a novel synthetic gene regulatory network. The aim of this circuit is to behave as a bistable switch for in vivo delivery of short hairpin RNA (shRNA) which can induce RNA interference (RNAi) of a target mRNA. Through the mathematical analysis performed here, it will be shown how the circuit can be controlled to induce sustained expression of a shRNA, with a transient input of two different inducer molecules.
Keywords :
cellular biophysics; genetics; molecular biophysics; patient treatment; bistable switch; induced RNA interference; inducible gene expression; mRNA; mammalian systems; mathematical model; shRNA in vivo delivery; short hairpin RNA; sustained shRNA expression; synthetic gene regulatory network; synthetic network; Bifurcation; Drugs; Integrated circuit modeling; Mathematical model; Proteins; RNA; Switching circuits;
Conference_Titel :
Biomedical Circuits and Systems Conference (BioCAS), 2010 IEEE
Conference_Location :
Paphos
Print_ISBN :
978-1-4244-7269-7
Electronic_ISBN :
978-1-4244-7268-0
DOI :
10.1109/BIOCAS.2010.5709601
