DocumentCode :
2471854
Title :
Increased cell killing and DNA damage in cells exposed to ultra-short pulsed electric fields
Author :
Stacey, M. ; Stickley, J. ; Fox, P. ; O´Donnell, Colm ; Schoenbach, K. ; Beebe, S. ; Buescher, S.
Author_Institution :
Center for Pediatric Res., Eastern Virginia Med. Sch., Norfolk, VA, USA
fYear :
2002
fDate :
20-24 Oct. 2002
Firstpage :
79
Lastpage :
82
Abstract :
We investigated the effects of nanosecond pulsed electric fields (nsPEF) on DNA structure and on survival of two human leukemia cell lines (Jurkat and HL60) in vitro. DNA damage was evaluated using single cell gel electrophoresis methods (comet assay) and sensitivity of cells to nsPEF determined by clonogenic cell survival. nsPEF applications using pulse durations of 10, 60 or 300 nanosecond duration with voltage amplitudes of 300, 60 and 60kv/cm respectively were applied to cells in trains of 1, 5, or 10 pulses. Jurkat and HL60 cells both showed DNA damage following exposure to different nsPEF parameters. Comet length increased in Jurkat cells by 22.5% at one, 33.2% at five and 62% at ten 60ns pulses compared to non-exposed cells. HL60 showed increases in comet length of 28.6%. 23.5% and 41.2% after one pulse of 10, 60 and 300ns duration, respectively. Cell survival curves for both cell types showed a decrease (90+/-4%) in cell survival following one 60 or 300ns pulse. A further dramatic decrease in cell survival was not observed following 5 and 10 pulses. These data show that exposure to nsPEF applications induces DNA damage and decreases cell survival in vitro.
Keywords :
DNA; bioelectric phenomena; biological effects of fields; biological techniques; biomolecular effects of radiation; blood; cancer; cellular effects of radiation; electrophoresis; 10 ns; 300 ns; 60 ns; DNA damage; DNA structure; HL60 cells; Jurkat cells; cell killing; cell survival in vitro; cell survival. curves; clonogenic cell survival; comet assay; comet length; human leukemia cell lines; nonexposed cells; pulse durations; single cell gel electrophoresis methods; trains; ultra-short pulsed electric fields; voltage amplitudes; Biomembranes; Cells (biology); DNA; Electrokinetics; Electromagnetic fields; Humans; In vitro; Microwave devices; Nanobioscience; Voltage;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Electrical Insulation and Dielectric Phenomena, 2002 Annual Report Conference on
Print_ISBN :
0-7803-7502-5
Type :
conf
DOI :
10.1109/CEIDP.2002.1048741
Filename :
1048741
Link To Document :
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