• DocumentCode
    2471929
  • Title

    A New method for viability identification of larvae 3–4 of Meloidogyne

  • Author

    Ma, Chao ; Zhang, YuXiu ; Wang, XinRong ; Gao, Hua ; Ren, LuLu

  • Author_Institution
    Lab. of Plant nematology, South China Agric. Univ., Guangzhou, China
  • fYear
    2011
  • fDate
    24-26 June 2011
  • Firstpage
    6596
  • Lastpage
    6600
  • Abstract
    A new method for differentiating live larvae 3-4 of M. incognita from dead one by 2%Trypan blue staining for 30 min is established. A live larvae 3-4 can not be stained, while the 1/3 body of the dead one is stained as blue. It is 100% effective for staining dead larvae 3-4 of M. incognita killed by either 601 for 3mins , -30°C for 30min, or 10-3g/L Abamectin. Trypan blue is firstly applied as a staining agent in nematode viability identification.
  • Keywords
    biochemistry; biological tissues; cellular biophysics; molecular biophysics; Abamectin; Meloidogyne incognita; Trypan blue staining; larvae 3-4; nematode viability identification; temperature -30 degC; time 3 min; time 30 min; Agriculture; Diseases; Fluorescence; Microscopy; Plants (biology); Taxonomy; Trypan blue; larvae 3–4 of Meloidogyne incognita; viability identification;
  • fLanguage
    English
  • Publisher
    ieee
  • Conference_Titel
    Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
  • Conference_Location
    Nanjing
  • Print_ISBN
    978-1-4244-9172-8
  • Type

    conf

  • DOI
    10.1109/RSETE.2011.5965871
  • Filename
    5965871
    • Link To Document
    https://search.isc.ac/dl/search/defaultta.aspx?DTC=49&DC=2471929