Title :
A New method for viability identification of larvae 3–4 of Meloidogyne
Author :
Ma, Chao ; Zhang, YuXiu ; Wang, XinRong ; Gao, Hua ; Ren, LuLu
Author_Institution :
Lab. of Plant nematology, South China Agric. Univ., Guangzhou, China
Abstract :
A new method for differentiating live larvae 3-4 of M. incognita from dead one by 2%Trypan blue staining for 30 min is established. A live larvae 3-4 can not be stained, while the 1/3 body of the dead one is stained as blue. It is 100% effective for staining dead larvae 3-4 of M. incognita killed by either 601 for 3mins , -30°C for 30min, or 10-3g/L Abamectin. Trypan blue is firstly applied as a staining agent in nematode viability identification.
Keywords :
biochemistry; biological tissues; cellular biophysics; molecular biophysics; Abamectin; Meloidogyne incognita; Trypan blue staining; larvae 3-4; nematode viability identification; temperature -30 degC; time 3 min; time 30 min; Agriculture; Diseases; Fluorescence; Microscopy; Plants (biology); Taxonomy; Trypan blue; larvae 3–4 of Meloidogyne incognita; viability identification;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5965871
