DocumentCode :
2472467
Title :
Cloning and Identification of lilyturf (Ophiopogon japonicus) RbcL cDNA fragment
Author :
Guo, Tianqi ; Li, Cong ; Wang, Yingbo ; Han, Liebao ; Zhang, Wenpan
Author_Institution :
Turf Grass Res. Inst., Beijing Forestry Univ., Beijing, China
fYear :
2011
fDate :
24-26 June 2011
Firstpage :
6682
Lastpage :
6685
Abstract :
This study was aimed to isolate ribulose-1,5-bisphosphate carboxylase/oxygenase large subunit (rbcL) from lilyturf (Ophiopogon japonicus). A pair of primers used in amplifying chloroplast fragment from lilyturf was designed according to the corresponding sequences in tobacco, spinach chloroplast genomes. The fragment containing rbcL gene was cloned from lilyturf chloroplast genome. Sequencing analysis indicated that this fragment was 800 base pairs (bp) in size. The BLAST results showed that the homologous amino acid coded by this gene fragment with tobacco, spinach, Convallaria majalis, Yucca schidigera, Nothoscordum inutile and Iris missouriensis were from 93% to 99%. Based on the newly identified cDNA sequence, multiple alignment was performed by using bioinformatics software among amino acid sequences of lilyturf and other higher plants. The cloning of rbcL gene is helpful for the further research of photosynthetic efficiency in lilyturf.
Keywords :
DNA; bioinformatics; botany; cellular biophysics; enzymes; genetics; molecular biophysics; photosynthesis; Convallaria majalis; Iris missouriensis; Nothoscordum inutile; Ophiopogon japonicus; Yucca schidigera; amino acid sequences; bioinformatics software; chloroplast fragment; cloning; homologous amino acid; identification; lilyturf RbcL cDNA fragment; photosynthetic efficiency; plants; ribulose-1,5-bisphosphate carboxylase-oxygenase large subunit; sequencing analysis; spinach chloroplast genome; tobacco genome; Amino acids; Biotechnology; Cloning; DNA; Genomics; Iris; RNA; chloroplast gene; lilyturf; rbcL gene; sequence analyses;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
Type :
conf
DOI :
10.1109/RSETE.2011.5965892
Filename :
5965892
Link To Document :
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