Cloning of DREB gene from Lilyturf (Ophiopogon japonicus) and construction of a plant expression vector

Author

Li, Cong ; Guo, Tianqi ; Li, Feifei ; Bao, Yongxia ; Han, Liebao

Author_Institution

Turf Grass Res. Inst., Beijing Forestry Univ., Beijing, China

fYear

2011

fDate

24-26 June 2011

Firstpage

6686

Lastpage

6689

Abstract

The cDNA of DREB transcription factor named OjDREBl was obtained from Lilyturf (Ophiopogon japonicus) under the drought stress of 12h by RT-PCR method. Sequence analysis showed that cDNA of OjDREBl is 1779 bp long, the open reading frame is 1062bp.The predicted protein has 353 amino acids with an estimated molecular mass of 38.69 kDa and an isoelectric point of 6.46. The deduced primary structure of OjDREBl contains a single AP2 domain and shows the typical characteristics of DREB gene family. Homological analysis showed that 73% amino acids in the sequences is similar to Asparagus officinalis DREB3. The OjDREBl gene was cloned into the PBI121 vector to obtain plant expression vector .

Keywords

DNA; genetics; molecular biophysics; molecular weight; proteins; DREB gene cloning; DREB transcription factor; Lilyturf; OjDREB1 gene; Ophiopogon japonicus; PBI121 vector; RT-PCR method; amino acids; cDNA; drought stress; homological analysis; isoelectric point; molecular mass; plant expression vector; primary structure; protein; sequence analysis; single AP2 domain; time 12 h; Amino acids; Cloning; DNA; Encoding; Proteins; RNA; Stress; DREB; Gene clone; Ophiopogon japonicus; Plant expression vector;

fLanguage

English

Publisher

ieee

Conference_Titel

Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on

Conference_Location

Nanjing

Print_ISBN

978-1-4244-9172-8

Type

conf

DOI

10.1109/RSETE.2011.5965893

Filename

5965893