Isolation of flower inducing gene SbCO in sorghum and construction of an inducible SbCO expression vector

Author

Lv, Fangfang ; Xie, Xiaodong ; Ding, Bo ; Yang, Wenli ; Zhong, Silin ; Sun, Shoujun

Author_Institution

TianJin Agric. Univ., Tianjin, China

fYear

2011

fDate

24-26 June 2011

Firstpage

6911

Lastpage

6916

Abstract

We isolated the coding sequence a putative SbCO gene from Sorghum cultivar, Roma, by in silico sequence analysis and subsequently, reverse-transcription PCR. The DNA sequencing data revealed 92% similarity between SbCO and the Arabidopsis flowering time regulator CONSTANS. To investigate the biological function of SbCO in sorghum, we have constructed an inducible expression vector for SbCO based on Gateway cloning technology. This study lays a solid foundation for future analysis of flowering time control in Sorghum.

Keywords

DNA; biochemistry; genetics; molecular biophysics; Arabidopsis flowering time regulator CONSTANS; DNA sequencing data; Sorghum cultivar; biological function; coding sequence; flower inducing gene SbCO; gateway cloning technology; inducible SbCO expression vector; putative SbCO gene; reverse-transcription PCR; Biological system modeling; Cloning; Encoding; Logic gates; Proteins; RNA; Expression vector; Gateway; SbCO gene; Sorghum;

fLanguage

English

Publisher

ieee

Conference_Titel

Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on

Conference_Location

Nanjing

Print_ISBN

978-1-4244-9172-8

Type

conf

DOI

10.1109/RSETE.2011.5965953

Filename

5965953