Title :
Purification of wheat esterase for detection of organophosphorus pesticide by a four-step extraction
Author :
Bin Jiang ; Zhibiao Feng ; Liying Lu
Author_Institution :
Dept. of Appl. Chem., NEAU, Harbin, China
Abstract :
A new method for purification of wheat esterase was build which include four steps. The wheat esterase was purified to electrophoretically pure through ammonium sulfate saturation, ultrafiltration, Sephadex G150 and Procainamide affinity chromatography. The wheat esterase was puried 127 fold with specific activity 1264 U/mg after the four steps. The molecular weight of the wheat esterase estimated was 45kDa by SDS-PAGE.
Keywords :
chromatography; purification; ultrafiltration; Procainamide affinity chromatography; Sephadex G150; ammonium sulfate saturation; four-step extraction; molecular weight; organophosphorus pesticide; ultrafiltration; wheat esterase purification; Biomedical optical imaging; Carbon; Optical films; Optical sensors; Physiology; Proteins; Purification; Procainamide affinity chromatography; Sephadex G150; purification; wheat esterase;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5965982
