Studies on the optimum technology for extraction of polysaccharides from Dendrobium candidum and which effects of the expression of NF-κB under induction and cultivation of high glucose

Conditions of the extraction of polysaccharides from protocorms of Dendrobium candidum were optimized by orthogonal experiment. The results showed that the optimum technology of isolation is to extract about 3 times and 0.5 hours each time in 30 times water at 100°C, the ways to break cell is high-speed homogenate. After 24h, the mRNA expressions of NF-κB was detected by reverse transcription-polymerase chain reaction (RT-PCR). The results suggested that Polysaccharides from Dendrobium candidum can decline the mRNA expressions of NF-κB in the vascular endothelial cells (ECV304) when it´s induction and cultivation under high glucose.