Study on biotransformation of inorganic selenium from Ganoderma lucidum and yeasts

The optimal conditions for shake flask were carried out by a single factor test and orthogonal test L₉(3⁴), giving a comparative study on the ability of biotransformation of inorganic selenium between Ganoderma lucidum and yeasts. The orthogonal experimental results showed that the optimal conditions for shake flask growth of Ganoderma lucidum were as bellow: culture medium final concentration of Na₂SeO₃ (Se⁴⁺) was 20mg/L, 15% inoculum, incubation time 42h, medium volume 60ml/250ml. The total content of selenium in fermentation of Ganoderma lucidum was 1540.34±19.21μg/g, and the growth capacity of mycelium of Ganoderma lucidum was 51.56±0.065g/L, respectively. The optimal conditions for shake flask cell growth of yeasts were as bellow: culture medium final concentration of Na₂SeO₃ (Se⁴⁺) was 25mg/L, 10% inoculum, incubation time 48h, medium volume 50ml/250ml. The total content of selenium in fermentation of yeasts was 2454.86±29.82 μg/g, and the growth capacity of cell of yeasts was 6.44±0.023 g/L, respectively.