Title :
Study on biotransformation of inorganic selenium from Ganoderma lucidum and yeasts
Author :
Huang, Shuming ; Zhang, Jie ; Zhang, Min ; Shen, Shouguo ; Yin, Xiaoyan ; Shui, Xiaoyan ; Wang, Dayan
Author_Institution :
Dept. of Biol. & Environ. Eng., Hefei Univ., Hefei, China
Abstract :
The optimal conditions for shake flask were carried out by a single factor test and orthogonal test L9(34), giving a comparative study on the ability of biotransformation of inorganic selenium between Ganoderma lucidum and yeasts. The orthogonal experimental results showed that the optimal conditions for shake flask growth of Ganoderma lucidum were as bellow: culture medium final concentration of Na2SeO3 (Se4+) was 20mg/L, 15% inoculum, incubation time 42h, medium volume 60ml/250ml. The total content of selenium in fermentation of Ganoderma lucidum was 1540.34±19.21μg/g, and the growth capacity of mycelium of Ganoderma lucidum was 51.56±0.065g/L, respectively. The optimal conditions for shake flask cell growth of yeasts were as bellow: culture medium final concentration of Na2SeO3 (Se4+) was 25mg/L, 10% inoculum, incubation time 48h, medium volume 50ml/250ml. The total content of selenium in fermentation of yeasts was 2454.86±29.82 μg/g, and the growth capacity of cell of yeasts was 6.44±0.023 g/L, respectively.
Keywords :
biotechnology; fermentation; food products; Na2SeO3; biotransformation; fermentation; ganoderma lucidum; incubation time; inorganic selenium; orthogonal test; shake flask cell growth; time 42 h; time 48 h; yeasts; Bellows; Educational institutions; Food industry; Genetics; Raw materials; Ganoderma lucidum (Fr.) Karst; Saccharomyces cerevisiae; orthogonal experiment; selenium-enriched; single-factor test;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5966007