Study on the galactose oxidase biosensor based on SiO2 sol-gel

Author

Li, Tong ; Zhao, Chun ; Liu, Baoyou

Author_Institution

Coll. of Environ. Sci. & Eng, Hebei Univ. of Sci. & Technol., Shijiazhuang, China

fYear

2011

fDate

24-26 June 2011

Firstpage

7153

Lastpage

7156

Abstract

A galactose oxidase biosensor is prepared based on the Prussian Blue(PB) modified electrode combined with SiO₂ sol-gel immobilization method in a "sandwich configuration". Its electrochemical characterization and related experiment condition have been studied in detail. The optimin detection potential is -100mV at pH 7.0, 25 °C.Under selected condition the sensor offered high sensitivity of 0.127μA (mmol·L^-1)^-1, a linear range of 0-18 mmol·L^-1 and a detection limit of 0.03mmol·L^-1 ,The response time is 10s. Both stability and selectivity of the biosensor are ideal.

Keywords

biosensors; electrochemical analysis; electrochemical electrodes; silicon compounds; sol-gel processing; SiO₂; electrochemical characterization; galactose oxidase biosensor; optimum detection potential; prussian blue modified electrode; sandwich configuration; sol-gel immobilization; temperature 25 degC; time 10 s; voltage -100 mV; Biochemistry; Biosensors; Electric potential; Electrodes; Industries; Time factors; Transducers; Enzyme biosensor; Galactose oxidase; Prussian blue; SiO2 sol-gel;

fLanguage

English

Publisher

ieee

Conference_Titel

Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on

Conference_Location

Nanjing

Print_ISBN

978-1-4244-9172-8

Type

conf

DOI

10.1109/RSETE.2011.5966015

Filename

5966015