Title :
Study on the interaction between rare earth complex [Er(C18H22.5N2O4)2] and bovine serum albumin
Author :
Xia, Hai-Tao ; Liu, Yu-Fen ; Rong, De-Fu
Author_Institution :
Sch. of Chem. Eng., Huaihai Inst. of Technol., Lianyungang, China
Abstract :
The interaction of rare earth complex [Er(C18H22.5N2O4)2] and bovine serum albumin (BSA) under physiological condition was studied by fluorescence spectrum. The experiment demonstrated that the fluorescence quenching of BSA by Er(III) complex is a result of the formation of ground state complex and the quenching mechanism is mainly static quenching. The corresponding binding constants between Er(III) complex and BSA were 5.17×103 L.mol-1(293K), and the binding sites were 1. According to the binding constants of the different temperatures, the thermodynamic parameters AH, AG, AS were calculated. From thermodynamic parameters it can be judged that the binding force between Er(III) complex and BSA is mainly H-bond and Van der Waals force. The effect of Er(III) complex on the conformation of BSA was analyzed by synchronous fluorescence spectrometry.
Keywords :
biochemistry; bioluminescence; erbium compounds; fluorescence; hydrogen bonds; molecular biophysics; organometallic compounds; proteins; radiation quenching; van der Waals forces; Er(III) complex; H-bond; binding constants; binding force; binding sites; bovine serum albumin conformation; fluorescence quenching; fluorescence spectrum; ground state complex formation; physiological condition; quenching mechanism; rare earth complex; static quenching; synchronous fluorescence spectrometry; temperature 293 K; thermodynamic parameters; van der Waals force; Biochemistry; Bovine; Fluorescence; Force; Land surface temperature; Spectroscopy; Thermodynamics; Bovine serum albumin; Fluorescence spectrum; Rare earth complex;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5966017