Title :
Studying effect of homologous protein as impurity on protein crystallization
Author_Institution :
Key Lab. of Microgravity (Nat. Microgravity Lab.), Inst. of Mech., Beijing, China
Abstract :
Homologous protein, which exists in protein solution, is an impurity during protein crystallization. We chose homemade fluorescein isothiocyanate (FITC) labeled hen egg white lysozyme (HEWL) as model homologous lysozyme impurity. We studied the effect of fluorescein isothiocyanate labeled hen egg white lysozyme (FITC-HEWL) on HEWL crystallization. We found that the existence of FITC-HEWL obviously delayed the appearance of HEWL crystals, which indicated that FITC-HEWL changed the nucleation process. At the same time, the amount of final formed HEWL crystals decreased as increase of FITC-HEWL concentration in HEWL crystal growth solution. Moreover, we found that the crystal {110} face growth rate decreased approximately 90% even if only 0.02mg/ml FITC-HEWL existed in HEWL crystal growth solution.
Keywords :
biochemistry; crystal growth; crystal structure; crystallisation; impurities; molecular biophysics; nucleation; proteins; HEWL crystal growth solution; HEWL crystallization; crystal face growth rate; fluorescein isothiocyanate labeled hen egg white lysozyme; homologous lysozyme impurity; homologous protein; nucleation process; protein crystallization; protein solution; Crystallization; Face; Impurities; Laboratories; Proteins; Springs; FITC; HEWL; impurity; protein crystallization;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5966028
