Title :
Eukaryotic expression of Porcine BST-2 gene and identification of biological activity of BST-2
Author :
Zhang, Tinghong ; Zhang, Zhenjie ; Zhao, Xie ; Chang, Weishan
Author_Institution :
Coll. of Animal Sci. & Technol., Shandong Agric. Univ., Taian, China
Abstract :
BST-2 is a new kind of antivirus protein that is induced by IFN α/ β. The BST-2 gene fragment was amplified by RT-PCR from pig kidney cells (PK-15) and subcloned into the pLenti7.3/V5-TOPO vector and the positive clone was filtrated. The positive plasmids were transfected into the HEK293T cells. The expressed protein was analyzed by SDS-PAGE. Vesicular stomatitis virus (VSV)-CEF restrain experiment and Porcine reproductive and respiratory syndrome virus (PRRSV) intervence experiment were carried out. Results showed that the sequence of BST-2 gene amplified by RT-PCR was the same as the sequence in gene map of GenBank and its protein molecular weight was 23 kD, which was the same as the fusion protein V5 BST-2, and the recombinant BST-2 has obvious antiviral activity.
Keywords :
antibacterial activity; cellular biophysics; genetics; kidney; microorganisms; proteins; GenBank; HEK293T cells; PRRSV intervence experiment; RT-PCR; SDS-PAGE; antiviral activity; antivirus protein; biological activity identification; eukaryotic expression; fusion protein; pLenti7.3/V5-TOPO vector; pig kidney cells; porcine BST-2 gene; porcine reproductive and respiratory syndrome virus; positive plasmids; protein molecular weight; vesicular stomatitis virus; Cells (biology); Embryo; Fibroblasts; Immune system; Kidney; Media; Proteins; BST-2; PRRSV; antiviral activity; porcine;
Conference_Titel :
Remote Sensing, Environment and Transportation Engineering (RSETE), 2011 International Conference on
Conference_Location :
Nanjing
Print_ISBN :
978-1-4244-9172-8
DOI :
10.1109/RSETE.2011.5966124
