Genetic diversity and relationship in 47 accessions of tomato by AFLP markers

The genetic diversity and relationship of 47 accessions of tomato from Russia, Japan, Italy and China were analyzed using 10 informative primer pairs of AFLP markers. Selective amplification detected 575 bands, of which 384 were polymorphic, accounting for 66.78% of the total bands. The number of amplified fragments and polymorphic fragments per primer combination were 57.5 and 38.4, respectively. The number of DNA bands per primer pair was ranging from 20 to 89, with a maximum polymorphic rate of 89.89% and a minimum rate of 49.21%. The genetic similarity among all 47 accessions evaluated ranged from 0.7200 to 0.9357. Based on the UPGMA dendrogram, all the materials could be divided into three types at a similarity coefficient of 0.80. The first group included 2 accessions, i.e. N-3 and M-16 introduced from Russia; The second group included 44 accessions, which could be divided into two subsets at a similarity coefficient of 0.82, 19 belonged to subset I , and the others to subset II; The third group included only one accession Jianhuang.