Studies on plant regeneration via organogenesis from Perilla frutescens

A high frequency plantlet regeneration system of Perilla frutescens was established from cotyledon and hypocotyl explants. A maximum of 91.1 % cotyledon and 76.4% of hypocotyl explants could directly produce shoots on Murashige and Skoog (MS) medium. The optimum hormone combinations were 1.0mg/L 6-benzylaminopurine (BA) for cotyledon and 0.5 mg/L BA+0.5 mg/L indole-3-acetic acid (IAA) for hypocotyls, respectively. Rooting was induced on half-strength hormone-free MS medium. After transplantation to soil, approximate 80% of the regenerated plantlets could survive, flower and fruits.