Title :
Using microelectrode models for real time cell-culture monitoring
Author :
Yúfera, Alberto ; Daza, Paula ; Cañete, Daniel
Author_Institution :
Seville Microelectron. Inst. (IMSE-CNM), Seville Univ., Seville, Spain
fDate :
Aug. 30 2011-Sept. 3 2011
Abstract :
This paper proposes a cell-microelectrode model for cell biometry applications, based on the area overlap as main parameter. The model can be applied to cell size identification, cell count, and their extension to cell growth and dosimetry protocols. Experiments performed with comercial electrodes are presented, illustrating a procedure to obtain cell number in both growth and dosimetry processes. Results obtained for the AA8 cell line are promising.
Keywords :
dosimetry; electrolytes; microelectrodes; AA8 cell line; cell biometry; cell dosimetry protocols; cell growth; electrolytes; microelectrode models; real time cell-culture monitoring; Biological system modeling; Electrodes; Frequency measurement; Impedance; Impedance measurement; Integrated circuit modeling; Resistance; ECIS; Microelectrode; bio-impedance; cell culture; dosimetry; impedance sensor; Animals; Cells, Cultured; Cricetinae; Microelectrodes; Models, Theoretical;
Conference_Titel :
Engineering in Medicine and Biology Society, EMBC, 2011 Annual International Conference of the IEEE
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-4121-1
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2011.6090989