Title :
A nanoelectromechanical biosensor based on precise quantification and control of DNA orientation
Author :
Spuhler, Philipp S. ; Sola, Laura ; Zhang, Xirui ; Monroe, Margo ; Greenspun, Joseph ; Chiari, Marcella ; Unlu, M. Selim
Author_Institution :
Dept. of Biomed. Eng., Boston Univ., Boston, MA, USA
fDate :
Aug. 30 2011-Sept. 3 2011
Abstract :
We utilize spectral self-interference fluorescent microscopy (SSFM) to measure fluorophore height with sub-nm precision to precisely quantify DNA orientation. A novel polymeric 3D scaffold is used to functionalize the sensor surface and to control orientation of the surface anchored DNA.
Keywords :
DNA; bioMEMS; biosensors; fluorescence spectroscopy; molecular biophysics; molecular configurations; nanoelectromechanical devices; optical microscopy; DNA orientation control; DNA orientation quantification; SSFM; fluorophore height; nanoelectromechanical biosensor; polymeric 3D scaffold; sensor surface functionalisation; spectral self interference fluorescent microscopy; surface anchored DNA; DNA; Electric fields; Fluorescence; Microscopy; Polymers; Probes; Proteins; Biosensing Techniques; DNA; Microscopy, Fluorescence;
Conference_Titel :
Engineering in Medicine and Biology Society, EMBC, 2011 Annual International Conference of the IEEE
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-4121-1
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2011.6091182
