DocumentCode
2489988
Title
A nanoelectromechanical biosensor based on precise quantification and control of DNA orientation
Author
Spuhler, Philipp S. ; Sola, Laura ; Zhang, Xirui ; Monroe, Margo ; Greenspun, Joseph ; Chiari, Marcella ; Unlu, M. Selim
Author_Institution
Dept. of Biomed. Eng., Boston Univ., Boston, MA, USA
fYear
2011
fDate
Aug. 30 2011-Sept. 3 2011
Firstpage
4774
Lastpage
4775
Abstract
We utilize spectral self-interference fluorescent microscopy (SSFM) to measure fluorophore height with sub-nm precision to precisely quantify DNA orientation. A novel polymeric 3D scaffold is used to functionalize the sensor surface and to control orientation of the surface anchored DNA.
Keywords
DNA; bioMEMS; biosensors; fluorescence spectroscopy; molecular biophysics; molecular configurations; nanoelectromechanical devices; optical microscopy; DNA orientation control; DNA orientation quantification; SSFM; fluorophore height; nanoelectromechanical biosensor; polymeric 3D scaffold; sensor surface functionalisation; spectral self interference fluorescent microscopy; surface anchored DNA; DNA; Electric fields; Fluorescence; Microscopy; Polymers; Probes; Proteins; Biosensing Techniques; DNA; Microscopy, Fluorescence;
fLanguage
English
Publisher
ieee
Conference_Titel
Engineering in Medicine and Biology Society, EMBC, 2011 Annual International Conference of the IEEE
Conference_Location
Boston, MA
ISSN
1557-170X
Print_ISBN
978-1-4244-4121-1
Electronic_ISBN
1557-170X
Type
conf
DOI
10.1109/IEMBS.2011.6091182
Filename
6091182
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