Wave shape classification of spontaneous neuronal activity in cortical cultures on micro-electrode arrays

Dissociated embryonal or postnatal rat cortical cells were cultured onto multi electrode arrays (MEA´s) with 61 electrode sites. They developed into networks and became spontaneously active after about one week in vitro. About 180,000 recorded action potential waveforms were sorted using several spike features and classified with a Mahalanobis distance sorting procedure. They were classified into six basic wave shapes.