Binarization of noisy microscopy images through signal reconstruction using iterative detection network

We propose a novel binarization method based on a signal reconstruction using an iterative detection network. The algorithm simulates the whole image acquisition process taking into account a point spread function of the imaging system and its noise characteristics. The negative influence of image blur and noise is effectively suppressed by iterative detection network based on the criterion of maximum a posteriori probability. The proposed method was successfully applied to noisy microscopy images. Experiments show that the proposed method due to the noise suppression and deconvolution properties provides for noisy images significantly better results compared to common thresholding techniques. Binarized images obtained by the proposed method can be particularly useful for particle detection and analysis of cell samples.