Neuronal adhesion and growth on polyethylenimine tracks microprinted on polyethylenoxide-polypropylenoxide coated surfaces

Author

Ruardij, T.G. ; van den Boogaart, M.A.F. ; Rutten, W.L.C.

Author_Institution

Dept. of Biomed. Eng., Twente Univ., Enschede, Netherlands

Volume

3

fYear

2002

fDate

23-26 Oct. 2002

Firstpage

2121

Abstract

Adsorbed layers of polyediylenoxide-polypropylenoxide (PEO-PPO) blockcopolymers were tested as neurophobic background coatings in neuronal patterning studies over a time period of 30 days. Microprinted tracks of polyethylenimine (PEI) were used as the neuron-adhesive part of the pattern. Results showed that PEO-PPO surfactants F108 and F127 (Synperonics, ICI) significantly reduced the growth of neuronal tissue when adsorbed on Polyimide and Fluorocarbon surfaces up till 8 days. Also survival of neuronal tissue was not negatively affected by the PEO-PPO. Viability of the cultures was assessed after 30 days and showed viability factors above 0.9 (scale 0 to 1).

Keywords

adhesion; biomedical materials; cellular biophysics; polymers; 30 d; F127; PEO-PPO surfactants F108; adsorbed layers; biofouling; cell-adhesive proteins; cultures viability; microprinted polyethylenimine tracks; multielectrode arrays; neuron-adhesive part; neuronal tissue survival; neurophobic background coatings; polyethylenoxide-polypropylenoxide coated surfaces; Adhesives; Biological materials; Biomedical engineering; Chemicals; Chemistry; Coatings; Neurons; Polyimides; Proteins; Testing;

fLanguage

English

Publisher

ieee

Conference_Titel

Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint

ISSN

1094-687X

Print_ISBN

0-7803-7612-9

Type

conf

DOI

10.1109/IEMBS.2002.1053198

Filename

1053198