Title :
Applications of Total Internal Reflection Fluorescence (TIRF) microscopy in cellular bio-imaging
Author :
Jin, L. ; Lee, R.K. ; Kong, S.K. ; Yuan, W. ; Ho, H.P. ; Lin, Chinlon
Author_Institution :
Chinese Univ. of Hong Kong, Hong Kong
Abstract :
Evanescent field based imaging and sensor technologies have been widely used for a long time, but only recently has TIRF been introduced for bio-imaging. This paper will discuss the reasons and give a brief summary of the recent development of TIRF which is also based on evanescent wave excitation, and which is well suited for ultra thin optical sectioning (hundreds of nanometres) at cell-substrate regions with an unusually high signal-to-noise ratio and image quality.
Keywords :
biological techniques; cellular biophysics; fluorescence; light reflection; optical microscopy; cell-substrate regions; cellular bioimaging; evanescent wave excitation; image quality; signal-to-noise ratio; total internal reflection fluorescence microscopy; ultra thin optical sectioning; Biomedical optical imaging; Biosensors; Fluorescence; Image sensors; Microscopy; Nanometers; Optical imaging; Optical reflection; Optical sensors; Optical surface waves;
Conference_Titel :
Optical Fiber Communication and Optoelectronics Conference, 2007 Asia
Conference_Location :
Shanghai
Print_ISBN :
978-0-9789217-2-9
Electronic_ISBN :
978-0-9789217-2-9
DOI :
10.1109/AOE.2007.4410884
