DocumentCode :
2493213
Title :
The effects of dye binding on calcium and dye diffusion
Author :
Wagner, John M.
Volume :
3
fYear :
2002
fDate :
23-26 Oct. 2002
Abstract :
Summary form only given. Fluorescent dyes used in dynamic measurements of cellular calcium signals have been found to bind to endogenous proteins. When these proteins are immobile or nearly immobile, dye binding hinders drastically the diffusion of the dye, and therefore, the diffusion of calcium. Beginning with a full model of calcium buffering and dye binding, we derive a simplified model based on the assumption of rapid local equilibrium. The dye is assumed to bind to immobile, endogenous proteins that hinder its diffusion, yet may still bind Ca2+ and fluoresce nominally even when bound. This model is then used to investigate the nature of both calcium and dye diffusion. We also explore conditions under which the simplified model remains valid by comparing it to the full model.
Keywords :
biochemistry; biodiffusion; calcium; dyes; fluorescence; molecular biophysics; proteins; Ca; buffering; dye binding effects; fluorescent dye; immobile endogenous proteins; modeling; protein binding; rapid equilibrium; rapid local equilibrium; Calcium; Fluorescence; Proteins;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Engineering in Medicine and Biology, 2002. 24th Annual Conference and the Annual Fall Meeting of the Biomedical Engineering Society EMBS/BMES Conference, 2002. Proceedings of the Second Joint
ISSN :
1094-687X
Print_ISBN :
0-7803-7612-9
Type :
conf
DOI :
10.1109/IEMBS.2002.1053250
Filename :
1053250
Link To Document :
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