Title :
Flow Type Electrochemical Immuno Sensing Systems
Author :
Yamaguchi, Yutaka ; Honda, Nobuo ; Inaba, Masashi ; Sekiguchi, Tetsushi ; Shoji, Shuichi
Author_Institution :
Waseda Univ., Tokyo
Abstract :
A novel flow type electrochemical immuno sensing system using 3-D comb electrodes, which enables high-speed enzyme immunoassay on a single microchip, has been developed. A trap-and-release system of magnetic beads and a sheath flow sorting system are integrated on the chip. The integrated system realizes repeatable sample preparation and sensing. Using this system, AFP detection in the range from 25 ng/mL to 500 ng/mL was obtained within 30 minutes. This system can be applied widely for high sensitive enzyme immunoassays.
Keywords :
biological techniques; chemical sensors; electrochemical analysis; enzymes; flow; lab-on-a-chip; microfluidics; 3D comb electrodes; AFP detection; enzyme immunoassay; flow type electrochemical immuno sensing; magnetic bead; sheath flow sorting system; trap-and-release system; Biochemistry; Electrodes; Ferrites; Immune system; Inductors; Optical sensors; Page description languages; Sensor systems; Sorting; System testing;
Conference_Titel :
Sensors, 2006. 5th IEEE Conference on
Conference_Location :
Daegu
Print_ISBN :
1-4244-0375-8
Electronic_ISBN :
1930-0395
DOI :
10.1109/ICSENS.2007.355512
