Title :
Estimation of the population of neutrophils induced to differentiate from the MPRO mouse promyelocytic cell line
Author :
Ivan, Fransiskus X. ; Welsch, Roy E. ; Chow, Vincent T K ; Rajapakse, Jagath C.
Author_Institution :
Singapore-MIT Alliance, Nanyang Technol. Univ., Singapore, Singapore
fDate :
Aug. 30 2011-Sept. 3 2011
Abstract :
Neutrophils derived from induced-differentiated mouse promyleocyte (MPRO) cell lines provide an alternative source of mouse neutrophils for in vitro experiments, substituting for primary mouse neutrophils that are normally obtained by sacrificing mice. One issue with using induced-differentiated MPRO cells (or NEUTs) is that they are usually composed of not only mature neutrophils, but also neutrophil precursors. Here, we report on an assessment of an automated image analysis system to estimate mature neutrophil proportion in giemsa-stained NEUTs, and compare the accuracy with manual cell counting and flow cytometry results.
Keywords :
cellular biophysics; image classification; image segmentation; medical image processing; MPRO mouse promyelocytic cell line; automated image analysis system; flow cytometry; giemsa-stained NEUT; in-vitro experiment; induced-differentiated mouse promyleocyte cell lines; manual cell counting; mature neutrophil proportion; mouse neutrophils; Blood; Educational institutions; Extracellular; Image analysis; Image segmentation; Manuals; Mice; Algorithms; Animals; Cell Count; Cell Differentiation; Cell Line; Cell Tracking; Flow Cytometry; Granulocyte Precursor Cells; Image Enhancement; Image Interpretation, Computer-Assisted; Mice; Neutrophils; Pattern Recognition, Automated; Reproducibility of Results; Sensitivity and Specificity;
Conference_Titel :
Engineering in Medicine and Biology Society, EMBC, 2011 Annual International Conference of the IEEE
Conference_Location :
Boston, MA
Print_ISBN :
978-1-4244-4121-1
Electronic_ISBN :
1557-170X
DOI :
10.1109/IEMBS.2011.6091483
