Chemiluminometric FIA system for Food Nutrient Analysis

A measurement system for food nutrient with flow injection analysis (FIA) system was constructed. The sensing system consisted of a peristaltic pump, a glucose oxidase (GOD) reactor and a photodiode. GOD was immobilized on a polytetrafluoroethylene (PTFE) membrane by crosslinking using glutaraldehyde. H₂O₂ generated by the GOD reaction oxidized luminol to produce chemiluminescence in the presence of horseradish peroxide. The chemiluminescence was detected using a photodiode. The calibration range of the nutrient sensor for glucose was from 2.0 to 100.0 mmol/1. The glucose concentration of tomato extraction measured with the glucose FIA system and the spectroscopy measurement were 78.4 mmol/1 and 94.4 mmol/1, respectively.