An Effective Combined-Enhancer for the PCR Amplification of GC-Rich Longer DNA Sequences

DNA manipulation technology is facing more challenges in the postgenomics era. The amplification of GC-rich longer DNA fragments PCR more difficult than others . through more and more enhancers have been investigated for their potential implications in developing better gene technology. In this study, we reported a beneficial effect of combined-enhancer in enhancing the specificity and total efficiency of GC-rich (GC% ges 65%) longer DNA fragments PCR. Combined-enhancer is a concentration-dependent combination of 5.5 M betaine ,0.5u pfu enzyme, 1.5 mM the mixture of 1,2- propylene glycol and ethylene glycol (the ration of 1,2- propylene glycol and ethylene glycol = 1:1)in 25 muL PCR condition. The combined-enhancer broadly enhanced the quantitative and/or qualitative output of GC-rich (GC% ges 65) longer DNA fragments PCRs.