An Efficient Microbial Membrane with Bacillus subtilis Applied in BOD Biosensor

In order to get some efficient microbial membrane, a Bacillus subtilis (QT2061) was isolated from wastewater, and was identified by the Micro Station System. It was used as the sensitive material for the BOD sensor (BODs) after being immobilized by PVA. Some practical wastewater samples and national standard BOD solutions were measured with the BODs compared with the traditional 5-day method. The result showed that the response time of the BOD sensor was only 8 min, furthermore, BODs could be kept stable for up to 10 days without significant loss in their activity. BOD in standard BOD solutions were estimated using the BODs with deviation within 5%. The BODs was also omnivorous to the real samples with a generally good agreement between the BOD values estimated by the biosensor and the conventional 5-day test.