DocumentCode :
2497865
Title :
Nanotube Molecular Probes: DNA Hybridization using Single Walled Carbon Nanotubes as Biomarkers
Author :
Cao, Chengfan ; Hong, Sanghyun ; Choi, Jae-Boong ; Kim, Young-Jin ; Baik, Seunghyun ; Hwang, Eung-Soo ; Jung, Hye-Jin ; Cha, Chang-Yong
Author_Institution :
Sungkyunkwan Univ., Suwon
fYear :
2006
fDate :
22-25 Oct. 2006
Firstpage :
1272
Lastpage :
1275
Abstract :
Single walled carbon nanotubes (SWNTs) hold particular advantage as optical nano-bio markers because they exhibit strong Raman signals. Such signals do not blink or photobleach under prolonged excitation. Here, new single-stranded DNA conjugated SWNT probes were developed to locate a particular sequence of DNA within a complex genome. Chromosomal DNAs of human fibroblast and Escherichia coli (E.coli) were used as a target and a control, respectively. Southern blotting, which uses photostable Raman signals of nanotubes instead of fluorescent dyes, demonstrated excellent sensitivity and specificity of the probes. The results showed SWNTs´ possibility of being generic nano-bio markers for precise detection of specific kinds of genes.
Keywords :
DNA; biosensors; carbon nanotubes; nanotube devices; probes; DNA hybridization; Escherichia coli; Raman signal; biomarkers; human fibroblast; nanotube molecular probes; optical nano-bio markers; single walled carbon nanotube; southern blotting; Bioinformatics; Biomarkers; Biomedical optical imaging; Carbon nanotubes; DNA; Genomics; Optical sensors; Photobleaching; Probes; Sequences;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Sensors, 2006. 5th IEEE Conference on
Conference_Location :
Daegu
ISSN :
1930-0395
Print_ISBN :
1-4244-0375-8
Electronic_ISBN :
1930-0395
Type :
conf
DOI :
10.1109/ICSENS.2007.355861
Filename :
4178856
Link To Document :
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