DocumentCode :
2498245
Title :
Analysis of Bacterial Community Structure in Vermifiltration for Sludge Reduction by Nested PCR-DGGE Method
Author :
Yang, Jian ; Li, Xiaowei ; Zhao, Limin ; Deng, Dehan ; Yi, Danghao ; Xing, Meiyan
Author_Institution :
State Key Lab. of Pollution Control & Resource Reuse, Tongji Univ., Shanghai, China
fYear :
2009
fDate :
11-13 June 2009
Firstpage :
1
Lastpage :
4
Abstract :
Vermiflltration was a innovative process to sludge reduction. The relationship of earthworm and bacterial community was not conclusive in the treatment. The techniques of polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE) was used to analyze the bacterial community structure in vermifiltration column in the paper. Results showed bacterial diversity index (H) in the middle layer was 1.87 and 3.19, and subsequently in the lower layer was 1.24 and 1.58. H in the upper layer was lowest (0.67 and 1.07). 11 bacterial organism were identified in the columns, demonstrated that richness and complexity of bacterial ecology. The important factors to determine bacterial community structure in vermifiltration was likely to be earthworm predation and cast, rather than bacterial community of raw sludge. Earthworm biomass reduced gradually in the depth distribution. The possible reasons were possible to be food sources and dissolved oxygen.
Keywords :
DNA; biochemistry; biological techniques; electrophoresis; enzymes; microorganisms; pattern clustering; sludge treatment; statistical analysis; wastewater treatment; PCR-DGGE method; bacterial community structure; bacterial diversity index; denaturing gradient gel electrophoresis; polymerase chain reaction; sludge reduction; vermifiltration; Biomass; Costs; Electrokinetics; Environmental factors; Microorganisms; Organisms; Pollution control; Polymer gels; Production; Wastewater treatment;
fLanguage :
English
Publisher :
ieee
Conference_Titel :
Bioinformatics and Biomedical Engineering , 2009. ICBBE 2009. 3rd International Conference on
Conference_Location :
Beijing
Print_ISBN :
978-1-4244-2901-1
Electronic_ISBN :
978-1-4244-2902-8
Type :
conf
DOI :
10.1109/ICBBE.2009.5162343
Filename :
5162343
Link To Document :
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